pcmv-mmlvgag-3xnes-abe8e-ng  (Addgene inc)

Journal: iScience

Article Title: Adenine base editing with engineered virus-like particles rescues the CFTR mutation G542X in patient-derived intestinal organoids

doi: 10.1016/j.isci.2025.111979

Figure Lengend Snippet:

Article Snippet: Plasmids used in this study include: pCMV-MMLVgag-3xNES-ABE8e-NG (Addgene plasmid #181754), a kind gift from David Liu; pBS-CMV-gagpol (Addgene plasmid #35614), a kind gift from Patrick Salmon; pCMV-VSV-G (Addgene plasmid #8454), a kind gift from Bob Weinberg; pU6-G542X-sgRNA-HEAT, custom-designed and purchased from Integrated DNA Technologies (IDT).

Techniques: Virus, Recombinant, Transfection, SYBR Green Assay, Amplification, Plasmid Preparation, Software

Journal: Cell

Article Title: Human T Cell Generation is Restored in CD3δ Severe Combined Immunodeficiency Through Adenine Base Editing

doi: 10.1016/j.cell.2023.02.027

Figure Lengend Snippet: C

Article Snippet: . pCMV-ABE8e-NG (Plasmid #138491) and pCMV-ABEmax-NRTH (Plasmid #136922) plasmids were obtained from AddGene (Watertown, MA).

Techniques: In Vivo, Purification, Calcium Flux Assay, Virus, Recombinant, Blocking Assay, Proliferation Assay, Cell Isolation, Cloning, CRISPR, Mutagenesis, Modification, Plasmid Preparation, Software

Journal: Cell

Article Title: Human T Cell Generation is Restored in CD3δ Severe Combined Immunodeficiency Through Adenine Base Editing

doi: 10.1016/j.cell.2023.02.027

Figure Lengend Snippet: C

Article Snippet: CD3δ SCID patient CD34+ HSPCs were cultured and electroporated with BE and sgRNA under the same conditions as CD34+ HSPCs isolated from healthy donors. . Cloning of Adenine Base Editor Variant Plasmids pCMV-ABE8e-NG (Plasmid #138491) and pCMV-ABEmax-NRTH (Plasmid #136922) plasmids were obtained from AddGene (Watertown, MA).

Techniques: In Vivo, Purification, Calcium Flux Assay, Virus, Recombinant, Blocking Assay, Proliferation Assay, Cell Isolation, Cloning, CRISPR, Mutagenesis, Modification, Plasmid Preparation, Software